Western leaders demanded explanations from Moscow on Wednesday after Germany said that Alexei Navalny was poisoned with a Novichok nerve agent in an attempt to “silence” the Russian opposition leader.

The 44-year-old last month became the latest in a long line of Russian defectors and critics of President Vladimir Putin to be poisoned in suspicious circumstances and is now in hospital in Berlin.

Medical tests from German military’s chemical weapons laboratory had given “unequivocal evidence” that Navalny had been afflicted by military-grade Novichok, a poison first developed by the Soviet Union towards the end of the Cold War.

German Chancellor Angela Merkel said the findings had prompted “some very serious questions that only Russia can and must answer”.

“Alexei Navalny is certainly the victim of a crime,” she added. “He was meant to be silenced and I condemn this in the strongest possible terms.”

The news prompted a fresh round of condemnation from European leaders in an affair that has already sent tensions spiralling between Russia and the West.

British Prime Minister Boris Johnson said Navalny’s poisoning was “outrageous”, while French Minister of Foreign Affairs Jean-Yves Le Drian denounced the attack “shocking and irresponsible”.

EU chief Ursula von der Leyen criticised a “despicable and cowardly act – once again”.

US National Security Council spokesman John Ullyot said: “The Russian people have a right to express their views peacefully without fear of retribution of any kind, and certainly not with chemical agents.”

NATO chief Jens Stoltenberg condemned the “shocking” use of a military-grade nerve agent which, he said, made “a full and transparent” investigation by Russia even more urgent.

Italy’s foreign ministry and Canadian foreign minister Francois-Philippe Champagne also condemned Navalny’s poisoning.

The nerve agent, whose name means “newbie” or “newcomer” in Russian, can be deployed in an ultra-fine powder, liquid or vapour.

It was famously used against ex-double agent Sergei Skripal in Britain in 2018, an assassination attempt that the West believes was ordered by the Kremlin despite Russia’s denials.

Navalny fell ill after boarding a plane in Siberia last month, with aides saying they suspect he drank a cup of spiked tea at the airport.

He was initially treated in a local Siberian hospital, where doctors said they were unable to find any toxic substances in his blood, before he was flown to Berlin for specialised treatment on August 22.

The Kremlin, which has previously questioned the credibility of German doctors, said Russia was ready to cooperate fully.

Russia’s embassy in Berlin said in a statement: “We call on our partners to avoid any politicisation of this incident and to rely solely on credible facts, which we hope will be delivered as soon as possible.”

The Charite hospital that has been treating Navalny reported improvements in his condition.

But the charismatic Yale-educated lawyer, who has been Russia’s leading opposition politician for around a decade, is still in the intensive care unit and remains on a ventilator.

Doctors say that over time the body can clear out the nerve agent, which disrupts communication between the brain, the main organs and muscles.

The hospital added: “Recovery is likely to be lengthy. It is still too early to gauge the long-term effects which may arise concerning this severe poisoning.”